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Protein expression systems make it very easy to sensitively detect various proteins used widely in bioanalytics and molecular biology. Fluorophores or certain vitamins are used to tag antibodies or other proteins, and these labelled molecules can then be purified or detected using other solutions such as streptavidin or monomeric avidin. These protein expression systems are optimized for the rapid synthesis of functionally active proteins.

Protein expression systems make it very easy to sensitively detect various proteins used widely in bioanalytics and molecular biology. Fluorophores or certain vitamins are used to tag antibodies or other proteins, and these labelled molecules can then be purified or detected using other solutions such as streptavidin or monomeric avidin. These protein expression systems are optimized for the rapid synthesis of functionally active proteins.


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Affinity protein expression and purification systems

Catalog Number: (AGLS214303)
Supplier: AGILENT
Description: The affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification.
UOM: 1 * 10 mL
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Freund's adjuvants

Supplier: Rockland Immunochemicals
Description: Freund's incomplete adjuvant

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TriLink® CleanCap® Renilla Luc mRNA (5 moU)

Supplier: TriLink BioTechnologies
Description: Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.

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ALiCE for Scale-Up

Supplier: LENIOBIO
Description: ALiCE® - Linearly scalable eukaryotic cell-free protein expression. Scalable protein expression with ALiCE® works as effectively for assessment of future development as it does in the exploration phase. The same simple protocol applies across all scales. Add the DNA sequence and let ALiCE® make your protein.

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TriLink® CleanCap® M6 EGFP mRNA (N1MePsU)

Supplier: TriLink BioTechnologies
Description: The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, <em>Aequorea victoria</em>. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

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TriLink® CleanCap® EGFP mRNA (5 moU)

Supplier: TriLink BioTechnologies
Description: The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, <em>Aequorea victoria</em>. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

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TriLink® CleanCap® FLuc mRNA

Supplier: TriLink BioTechnologies
Description: The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

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ALiCE for Research

Supplier: LENIOBIO
Description: From ALiCE® for Research to ALiCE® for Scale-Up Protein Expression, we offer cell-free Protein Expression products with radical results for every production setup.

New Product

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TriLink® CleanCap® M6 EPO mRNA (N1MePsU)

Supplier: TriLink BioTechnologies
Description: <p>EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA <em>in vivo</em> resulted in significant increases of both reticulocyte counts and hematocrits.</p>

New Product

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ProLite™ His-Tag Protein Gel Staining Kit

Catalog Number: (AATB18010)
Supplier: AAT BIOQUEST
Description: This kit requires very little hands-on time, allowing for rapid protein expression screening in a variety of gel types using an imaging equipment with a standard FITC filter.
UOM: 1 * 10 items
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Squalene emulsion, SqualVax

Supplier: OZ BIOSCIENCES
Description: SqualVax is an oil-in-water emulsion made of squalene droplets in a continuous aqueous phase. It is an important advantage over alternative oils that have been used in emulsion adjuvants, like Freund’s adjuvant that contains mineral oil (paraffin oil) and has long term persistence in organisms.

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Alum adjuvant, AlumVax hydroxide

Supplier: OZ BIOSCIENCES
Description: AlumVax hydroxide is a wet gel (colloidal) of aluminium hydroxide 2%. It is the most common adjuvant used in approved prophylactic vaccines because of its excellent safety profile and ability to enhance the protective humoral immune response.

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Freund's incomplete adjuvant, Imject™

Catalog Number: (PIER77145)
Supplier: Thermo Fisher Scientific
Description: Imject™ Freund's Incomplete Adjuvant (FIA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.
UOM: 1 * 1 SET
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Alum adjuvant, AlumVax phosphate

Supplier: OZ BIOSCIENCES
Description: AlumVax phosphate is a wet gel (colloidal) of aluminium phosphate 2%. Aluminium salts are the most common adjuvants used in approved prophylactic vaccines because of their excellent safety profile and ability to enhance the protective humoral immune response.

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Cell-free protein expression kits, 1-step human coupled IVT kit - DNA

Supplier: Thermo Fisher Scientific
Description: The 1-Step Human Coupled IVT Kit – DNA is a mammalian<i> in vitro</i> translation (IVT) system based on HeLa cell lysates. The kit contains all of the cellular components required for protein synthesis, including ribosomes, initiation factors, elongation factors and tRNA. When supplemented with the included proprietary accessory proteins, reaction mix and a DNA template cloned into the Thermo Scientific pT7CFE1-based vector, this system can synthesise protein for up to 6 hours. The kit includes a pT7CFE-CHis vector for ease of use in downstream applications.

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Freund's incomplete adjuvant, IFAVax

Supplier: OZ BIOSCIENCES
Description: Incomplete Freund’s Adjuvant (IFA) is a water-in-oil emulsion that is similar to the complete Freund’s Adjuvant (CFA) without addition of heat-killed mycobacteria (<i>Mycobacterum butyricum</i>).

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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