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You Searched For: Extraction Thimbles

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Product Image-PIER90067

Protein quantitation reagent, TMTzero™ label reagent

Description: Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.
Catalog Number: PIER90067
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-786-088

Carrier proteins

Description: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.
Catalog Number: 786-088
UOM: 1 * 10 mg
Supplier: G-Biosciences
Image Unavailable-786-214

Detergent removal system, DetergentOUT™ Tween®

Description: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.
Catalog Number: 786-214
UOM: 1 * 10 items
Supplier: G-Biosciences

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Product Image-786-128

Detergent removal systems, OrgoSol DetergentOUT™

Description: OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. Compatible with all detergent types, its performance is not dependent on the concentration of detergents in the solution. It is highly flexible and can be used to process small and large sample volumes.
Catalog Number: 786-128
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-PIER90051

Pierce™ EndoProteinase Lys-C Protease, MS Grade

Description: Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
Catalog Number: PIER90051
UOM: 1 * 20 µG
Supplier: Thermo Fisher Scientific
Product Image-PIER84841

Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific

Description: The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.
Catalog Number: PIER84841
UOM: 1 * 10 µG
Supplier: Thermo Fisher Scientific
Product Image-786-428

Adenine deaminase

Description: Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.
Catalog Number: 786-428
UOM: 1 * 0,25 mL
Supplier: G-Biosciences
Product Image-PIER20343

Immobilized pepsin, Pierce™

Description: Immobilised Pepsin agarose consists of an acidic endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.
Catalog Number: PIER20343
UOM: 1 * 5 mL
Supplier: Thermo Fisher Scientific
Product Image-PIER20341

Immobilised papain, Pierce™

Description: Pierce™ Immobilised Papain consists of a cysteine-endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
Catalog Number: PIER20341
UOM: 1 * 5 mL
Supplier: Thermo Fisher Scientific
Product Image-PIER87779

Pierce™ Detergent Removal Spin Columns and Resin

Description: Pierce™ Detergent removal resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions.
Catalog Number: PIER87779
UOM: 1 * 5 items
Supplier: Thermo Fisher Scientific
Product Image-PIER88305

Detergent removal spin column kit, HiPPR™

Description: The HiPPR™ Detergent Removal Spin Column Kit improves mass spectrometry results by efficiently removing detergents from 25 to 200 µl samples with low protein or peptide concentrations.
Catalog Number: PIER88305
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-ICNA116978050

Lysing matrix Z, MP Biomedicals

Description: Lysing Matrix Z is used primarily for lysis of animal and plant tissue, tougher stems, roots and seeds, and whole insects. Each impact-resistant tube contains yttria-stabilised zirconium oxide beads.
Catalog Number: ICNA116978050
UOM: 1 * 50 items
Supplier: MP Biomedicals
Product Image-PIER20308

SDS precipitation kit, SDS-Out™

Description: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.
Catalog Number: PIER20308
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-PIER88216

Micrococcal nuclease solution (≥100 U/µl)

Description: This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
Catalog Number: PIER88216
UOM: 1 * 1 items
Supplier: Thermo Fisher Scientific
Product Image-786-791

Immobilised Pepsin

Description: A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
Catalog Number: 786-791
UOM: 1 * 1 items
Supplier: G-Biosciences
Product Image-786-790

Immobilised Papain

Description: A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
Catalog Number: 786-790
UOM: 1 * 1 items
Supplier: G-Biosciences
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