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Description: Fluor de Lys® Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the Fluor de Lys® Developer produces a fluorophore. Histone deacetylases from all known classes have been successfully assayed with the Fluor de Lys® Substrate. These include human HDACs 1, 2, 3 and 8 (class I), human HDACs 4 and 9 (class II) and the sirtuins yeast Sir2, human SIRT1 and human SIRT2 (class III; reaction must include the sirtuin substrate NAD+). The substrate is cell-permeable and serves as the basis for the HDAC Cellular Activity Assay Kit (Prod. No. BML-AK503). NOTE: Must be used in conjunction with Fluor de Lys® Developer (Prod. No. BML-KI105). Sufficient for 100-1000 assays of HDACs or sirtuins (50 µl reaction volume, 50-500 µM (substrate).
Catalog Number: ENZOBMLKI1040050
UOM: 1 * 50 µl
Supplier: ENZO LIFE SCIENCES


Description: HDAC Substrate (fluorogenic)
Catalog Number: ENZOALX260137M005
UOM: 1 * 5 mg
Supplier: ENZO LIFE SCIENCES


Description: Fluorogenic substrate for several matrix metalloproteinases (MMPs).
Catalog Number: ENZOBMLP1280001
UOM: 1 * 1 mg
Supplier: ENZO LIFE SCIENCES


Description: This peptide, YIYGSFK, was derived through a random combinatorial peptide library method and is a specific and efficient substrate for the scr family of kinases. The Km of YIYGSFK for p60c-src is 55 µM.
Catalog Number: ENZOBMLP2590001
UOM: 1 * 1 mg
Supplier: ENZO LIFE SCIENCES


Description: This peptide, (Ac-S)-PGRRRRK-biotin, is a substrate for CDC2 kinase.
Catalog Number: ENZOBMLP2720001
UOM: 1 * 1 mg
Supplier: ENZO LIFE SCIENCES


Description: This peptide, Biotin-Ahx-PKTPKKAKKL, is derived from histone H1 and is a substrate for CDK5.
Catalog Number: ENZOBMLP2640001
UOM: 1 * 1 mg
Supplier: ENZO LIFE SCIENCES


Description: Concentrate diluent buffer for DAB membrane substrate, 10X
Catalog Number: ROCKDAB-B50
UOM: 1 * 50 mL
Supplier: Rockland Immunochemicals


Description: Fluorogenic substrate cleaved by plasma kallikrein and kallikrein 8, but not plasmin; by papain; and by most cathepsins (cathepsins B, C/DPP-I, F, K/O2, L, L2/V, O, S, and X/Z). Ex.: 380 nm, Em.: 460 nm, although the following Ex/Em can also be used: 355,375/440,450.
Catalog Number: ENZOBMLP1390010
UOM: 1 * 10 mg
Supplier: ENZO LIFE SCIENCES


Description: This peptide, Biotin-Ahx-GGMEDIYFEFMGGKKK, identified by screening a degenerate peptide library, is a substrate for several kinases, including HER-2/c-neu/erb-B-2, Tie-2, cFMS, and cKit.
Catalog Number: ENZOBMLP2430001
UOM: 1 * 1 mg
Supplier: ENZO LIFE SCIENCES


Description: 3,3',5,5'-Tetramethylbenzidine (TMB) EASY, liquid substrate
Catalog Number: ACRO328030300
UOM: 1 * 30 mL
Supplier: Thermo Fisher Scientific

MSDS


Description: This peptide, KEAPEDLYKDFLTL, is derived from the KDR sequence and is a substrate for FGFR.
Catalog Number: ENZOBMLP2440001
UOM: 1 * 1 mg
Supplier: ENZO LIFE SCIENCES


Description: This peptide sequence, EFpYAEVGRSPPDPAK, was selected by phage display analysis for optimal Shp-1 and Shp-2 phosphatase activities.
Catalog Number: ENZOBMLP1570001
UOM: 1 * 1 mg
Supplier: ENZO LIFE SCIENCES


Description: Magnesium oxide substrate 10x10x0.5mm, polished one side 110 orientation
Catalog Number: 40272.KU
UOM: 1 * 5 items
Supplier: Thermo Fisher Scientific

Description: Magnesium oxide substrate 10x10x0.5mm, polished two sides 100 orientation
Catalog Number: 41436.KT
UOM: 1 * 1 items
Supplier: Thermo Fisher Scientific

Description: Fluorogenic substrate for several matrix metalloproteinases (MMPs).
Catalog Number: ENZOBMLP1310001
UOM: 1 * 1 mg
Supplier: ENZO LIFE SCIENCES


Description: Fluorogenic substrate for TACE (ADAM17) (between Ala-Val), as well as MMP-3, MMP-7, MMP-12, and MMP-17, but not for ADAM19, MMP-13, nor MMP-14. Similar substrates are also cleaved by ADAM10, ADAM8 and ADAM9, and MMP-1, -2, and -9. This quenched substrate is useful for TACE inhibitor screening and kinetic analysis. Ex: 328 nm, Em: 393 nm, although the following Ex/Em have been used: 320-340/400-420. Also available: OMNIMMP® Fluorogenic Control peptide (BML-P127).
Catalog Number: ENZOBMLP1320500
UOM: 1 * 0,5 mg
Supplier: ENZO LIFE SCIENCES