Tween® 80 (Polysorbate), clear, yellow viscous liquid

Supplier: MP Biomedicals

0210317080 0210317090 0210317091
ICNA0210317080EA 33.8 EUR
ICNA0210317080 ICNA0210317090 ICNA0210317091
Tween® 80 (Polysorbate), clear, yellow viscous liquid
Tween® 80 (Polysorbate)

Tween 80 is a polyethylene sorbitol ester, non ionic surfactant and an emulsifier derived from polyethoxylated sorbitan and oleic acid.


  • Aggregation Number: 60
  • Critical Micelle Concentration (CMC): 0.012 mM (13-15 mg/L)


Tween 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture, growing of tubercule bacilli, and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent. It has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds.
Tweens® are a series of nonionic surfactants derived from sorbitan esters. They are soluble or dispersible in water but differ widely in organic and oil solubilities. Used as oil-in-water emulsifiers in pharmaceuticals, cosmetics, cleaning compounds, etc.


Tween 80 has been widely used in biochemical applications including: solubilising proteins, isolating nuclei from cells in culture, growing of tubercule bacilli, and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent. It has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds.


Soluble/miscible in water to give a clear yellow solution; miscible with alcohol, dioxane, and ethyl acetate; practically insoluble in liquid paraffin and fixed oils (such as mineral oil).


Caution: Research Use Only (RUO).

Boiling Pt: 100 °C (lit.)
Density: 1,08 g/mL (lit.)
Storage Temperature: Ambient
MDL Number: MFCD00082107
CAS Number: 9005-65-6

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Specification Test Results

Identity Test A Passes
Identity Test B Passes
Identity Test C Passes
Water Content ≤3.0%
Residue on Ignition ≤0.25%
Specific Gravity 1.06-1.09
Viscosity 300-500cps
Heavy Metals ≤100ppm
Acid Value ≤2.2
Hydroxyl Value 65-80
Saponification Value 45-55

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