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Product Image-BE-314

Experimentation kits, mutation detection and analysis

Catalog Number: (BE-314)
Supplier: G-Biosciences
Description: A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.
UOM: 1 * 1 KIT
Product Image-BE-406

Experimentation kits, protein electrophoresis

Catalog Number: (BE-406)
Supplier: G-Biosciences
Description: Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.
UOM: 1 * 1 KIT

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Product Image-EDVO212

Experimentation kits, cleavage of Lambda DNA with Eco RI endonuclease: intro to restriction enzymes

Catalog Number: (EDVO212)
Supplier: EDVOTEK
Description: The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for <i>Eco</i> RI (5 distinct sites, 2 are very close in size). In this experiment, Lambda DNA is digested by the <i>Eco</i> RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.
UOM: 1 * 1 KIT
Product Image-EDVO300

Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase

Catalog Number: (EDVO300)
Supplier: EDVOTEK
Description: When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent <i>E. coli</i> cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.
UOM: 1 * 1 KIT
Product Image-EDVO225

Experimentation kits, DNA fingerprinting using restriction enzymes

Catalog Number: (EDVO225)
Supplier: EDVOTEK
Description: This experiment helps teach students about restriction enzyme digests in the context of forensic science. Students cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.
UOM: 1 * 1 KIT

MSDS


Product Image-EDVO303

Experimentation kits, exploring biotechnology with green fluorescent protein (GFP)

Catalog Number: (EDVO303)
Supplier: EDVOTEK
Description: Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.
UOM: 1 * 1 KIT
Product Image-BE-308

Experimentation kits, DNA ligation

Catalog Number: (BE-308)
Supplier: G-Biosciences
Description: Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyses the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.
UOM: 1 * 1 KIT
Product Image-BE-402

Experimentation kits, assays for protein quantification

Supplier: G-Biosciences
Description: Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.

Image Unavailable-EDVO715

Restriction enzymes, Dryzymes®

Supplier: EDVOTEK
Description: The three most frequently used restriction enzymes are Eco RI, Bam HI and Hind III. Each enzyme catalyses cleavage at the defined base sequence. All enzymes are lyophilised and contain 1500 units. One unit is defined as the amount of enzyme required to digest 1,0 µg of lambda DNA in 60 minutes at 37 °C in a total reaction mixture of 50 µl.

Product Image-0694-4L

TRITON™ X-100 (Polyethylene glycol tert-octylphenyl ether), Reagent Grade for biochemistry

Supplier: VWR Chemicals
Description: Non-ionic detergent efficiently separates hydrophilic proteins from membrane spanning, hydrophobic proteins without altering biological activity.

MSDS Certificates

Product Image-RDBT002015

VWR® Electrode Holders

Catalog Number: (RDBT002015)
Supplier: VWR Chemicals
Description: Plain borosilicate tubes for insertion of a wire electrode so that the electrode is insulated from the clamp or holder and protected from mechanical damage. The tube also helps the flexible wire electrode be positioned correctly in a rigid support.
UOM: 1 * 100 items
Product Image-BE-502

Experimentation kits, dot blot analysis

Catalog Number: (BE-502)
Supplier: G-Biosciences
Description: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.
UOM: 1 * 1 KIT
Product Image-201-0216

Capillary tube on foot

Catalog Number: (201-0216)
Supplier: GLASWARENFABRIK KARL HECHT
Description: Five communicating glass tubes with different inner diameters. Used to show capillary forces and how they are affected by the inner diameter of the pipes.
UOM: 1 * 1 items
Product Image-765-0969

Communicating tubes

Catalog Number: (765-0969)
Supplier: GLASWARENFABRIK KARL HECHT
Description: Five glass tubes of different shape and inner dimensions. Down to each other. When filling the liquid in the pipes, it is clear that the height of the stowage in the tubes. pipes are independent of the design of the pipes. Comes with foot.
UOM: 1 * 1 items
Image Unavailable-BOSSBS-11719R-A488

Anti-CALY Rabbit Polyclonal Antibody (Alexa Fluor® 488)

Catalog Number: (BOSSBS-11719R-A488)
Supplier: Bioss
Description: Calcyon is a single transmembrane protein that interacts with D1 dopamine receptors. Dopamine is a neurotransmitter that regulates synaptic transmission involved in learning and memory. D1 receptors, the most abundant dopamine receptor in the central nervous system, appear to modulate the activity of D2 dopamine receptors, mediate various behavioural responses, and regulate neuron growth and differentiation. Calcyon is present in neuronal cell bodies and processes of the cortex and hippocampus, and it is especially abundant in pyramidal neurons. Interaction of Calcyon with D1 receptors results in a release of intracellular calcium.
UOM: 1 * 100 µl
Image Unavailable-BOSSBS-5382R-A350

Anti-GRIN2B Rabbit Polyclonal Antibody (Alexa Fluor® 350)

Catalog Number: (BOSSBS-5382R-A350)
Supplier: Bioss
Description: N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008].
UOM: 1 * 100 µl
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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