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Product Image-786-405

Hybridisation buffers, formamide-free, HYB-LINK™

Supplier: G-Biosciences
Description: Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.

Product Image-F-416XL

DyNAmo™ ColorFlash SYBR® green QPCR kits

Supplier: FINNZYMES REAGENTS
Description: DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

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ABsolute™ qPCR Mixes

Supplier: Thermo Fisher Scientific
Description: ABsolute™ qPCR Mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers. Versions are available for use with probes or with SYBR® Green I included, and with or without ROX or Fluorescein passive reference dyes.

Product Image-K0394

Luminaris color HiGreen qPCR master mix

Supplier: Thermo Fisher Scientific
Description: Luminaris Color HiGreen qPCR Master Mixes are universal ready to use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start <i>Taq</i> DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template and primers need to be added. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Product Image-F-415L

DyNAmo™ Flash SYBR® green qPCR kits

Supplier: FINNZYMES REAGENTS
Description: DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

Product Image-710-1499

Food safe kits for identification of pathogenic microorganisms

Supplier: BIOPREMIER
Description: Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of a specific pathogenic bacteria. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target microorganism. PCR is a method used to amplify a specific DNA sequence which is typically amplified in a reaction containing a thermostable DNA polymerase, nucleotides, and primers complementary to the target sequence. When this solution is heated, the DNA molecule denatures, separating into two strands. As the solution cools, the primers anneal to the target sequences in the separated DNA strands and the DNA polymerase synthesises a new strand by extending the primers with nucleotides, creating a copy of the DNA sequence (amplicons). When repeated, this cycle of denaturing, annealing, and extending exponentially increases the number of target amplicons. In Real-Time PCR, specific fluorescent probes are used to detect the amplified DNA by hybridising with amplicons. These probes are linked to a fluorophore on one end and a quencher that suppresses fluorescence on the other. If the target sequence is present during the PCR, amplification occurs and the probe is degraded, resulting in fluorescence increase. Fluorescence is measured by a detector and the associated software plots the fluorescence intensity versus number of cycles, allowing the determination of the presence or absence of the target organism.

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ABsolute™ qPCR Capillary Mixes

Supplier: Thermo Fisher Scientific
Description: ABsolute™ qPCR Capillary Mixes contain an optimised reaction buffer specifically designed to provide the highest possible sensitivity, specificity and consistent end-point values in real time instruments that use glass capillaries, such as the Roche LightCycler®. The 2X mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

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Maxima™ SYBR green/ROX QPCR master mix (2X)

Supplier: Thermo Fisher Scientific
Description: The Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer and is supplemented with ROX passive reference dye. Only template and primers need to be added. Maxima™ hot start <i>Taq</i> DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

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ABsolute™ qPCR Low ROX Mixes

Supplier: Thermo Fisher Scientific
Description: ABsolute™ qPCR low ROX mixes are optimised for use with real time instruments that are able to detect long wavelength dyes with greater sensitivity, such as the ABI 7500. The lower level of ROX still facilitates normalisation across the plate, while maintaining the sensitivity of the assay. The mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X qPCR mix contains a proprietary reaction buffer that provides high sensitivity, specificity and reproducibility.

Product Image-PRSI79-340

Anti-MAP2K6 Rabbit Polyclonal Antibody

Catalog Number: (PRSI79-340)
Supplier: ProSci Inc.
Description: MEK6 is a member of MAPKK protein kinase family. By using degenerate oligonucleotide primers from the conserved kinase domains of MKK3 and MKK4 two human cDNAs and 1 murine cDNA encoding closely related proteins of the MKK family were cloned. The two human clones appear to be different isoforms of the same gene generated by differential splicing: the shorter clone was designated MKK6, encodes a 278-amino acid protein, while the longer clone, designated MKK6b, encodes a 334-amino acid protein. MKK6 is about 80% identical to MKK3 and 40% identical to MKK4. 1.7-kb human MKK6 transcript is highly expressed in skeletal muscle, while an MKK6b-specific probe detected mRNA bands of 1.8, 2.4, and 4.5 kb that are enriched in heart, skeletal muscle, pancreas and liver. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase. MEK6 phosphorylates and activates p38 in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAPK mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
UOM: 1 * 100 µG
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IHC tissue primer

Catalog Number: (ENZOADI9502340100)
Supplier: ENZO LIFE SCIENCES
Description: Immunohistochemistry tissue primer is an enzyme blocking reagent.
UOM: 1 * 100 mL

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Product Image-733-2543

VWR®, Taq DNA polymerase master mix

Supplier: VWR Chemicals
Description: VWR® <i>Taq</i> DNA Polymerase Master Mix is a ready to use 1,1X or 2X reaction mix. Simply add primers, template and water to carry out primer extensions and other molecular biology applications.

MSDS Certificates

Product Image-N224-500U

VWR®, Taq DNA polymerase, 500 U

Catalog Number: (N224-500U)
Supplier: VWR Chemicals
Description: <i>Taq</i> DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity. PCR can be initiated from as few as 6 primed templates. Simply add 0,5 µl of <i> Taq</i> DNA Polymerase to a 25 µl PCR reaction.
UOM: 1 * 500 items

MSDS


Product Image-733-2450

Betaine enhancer solution 5 M (5X) for PCR

Supplier: VWR Chemicals
Description: Betaine enhancer is especially effective when used with high GC-rich regions or templates with a high degree of secondary structures. It has a decreasing effect on the primer melting temperature.

MSDS Certificates

Product Image-733-2479

VWR®, Taq DNA polymerase, 200000 U

Catalog Number: (733-2479)
Supplier: VWR Chemicals
Description: Taq DNA polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from thermus aquaticus and has a molecular weight of approximately 94 kDa.
UOM: 1 * 1 KIT
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VWR®, Taq DNA polymerase glycerol free

Supplier: VWR Chemicals
Description: VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.

MSDS

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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