You Searched For: Thermo Fisher Scientific

Catalog Number: (PIER37570)

Supplier: Thermo Fisher Scientific

Description: These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.

UOM: 1 * 1 L

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Supplier: Thermo Fisher Scientific

Description: Halt protease and phosphatase inhibitor cocktail provides the convenience of a single solution with full sample protection. This broad-spectrum inhibitor cocktail is specifically optimised to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases.

MSDS

Supplier: Thermo Fisher Scientific

Description: Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.

Supplier: Thermo Fisher Scientific

Description: Pierce™ low protein binding microcentrifuge tubes help to minimise protein sample loss during sample manipulation and are ideal in applications where processing steps are extensive.

Supplier: Thermo Fisher Scientific

Description: Protein A/G magnetic agarose beads provide a fast, convenient method for purification of immunoglobulins from serum, cell culture supernatant, or ascites.

Supplier: Thermo Fisher Scientific

Description: Streptavidin, horseradish peroxidase (HRP) conjugate includes streptavidin in a purified form conjugated to peroxidase for substrate-based detection.

Catalog Number: (PIERA35393)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

UOM: 1 * 10 mg

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Catalog Number: (PIER62242)

Supplier: Thermo Fisher Scientific

Description: This black plastic sample screen is used to cover the transilluminator on the myECL™ imager.

UOM: 1 * 1 items

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Supplier: Thermo Fisher Scientific

Description: These protein magnetic beads are high-capacity and high-throughput affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Catalog Number: (PIER37577)

Supplier: Thermo Fisher Scientific

Description: Pierce™ Fast Wash buffer, 10X, for use with Pierce™ Fast Blocking buffer.

UOM: 1 * 1 L

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Catalog Number: (PIER90115)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific 50% Hydroxylamine is a 10X quenching reagent for amine-reactive isobaric mass tag labeling experiments.

UOM: 1 * 5 mL

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Supplier: Thermo Fisher Scientific

Description: Transfer membranes made of high quality PVDF, which provides high binding capacity for proteins and nucleic acids.

Catalog Number: (PIER26175)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.

UOM: 1 * 50 mg

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Supplier: Thermo Fisher Scientific

Description: The methyl-PEG_n-amine (MA[PEG]_n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

Catalog Number: (PIER88828)

Supplier: Thermo Fisher Scientific

Description: The Direct Magnetic IP/Co-IP Kit uses NHS-chemistry to covalently immobilise IP antibodies onto magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: DyLight™ Fluor phosphine reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.