You Searched For: Pyrazinamide

Supplier: G-Biosciences

Description: Tween® 20 is commonly used non ionic detergent for solubilising membrane proteins during isolation of membrane-protein complexes.

Supplier: G-Biosciences

Description: Nonidet® P 40 Substitute (NP-40)

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Catalog Number: (DG521)

Supplier: G-Biosciences

Description: This variety pack contains non ionic proteomic grade detergent solutions, zwitterionic and non-detergent sulphobetaines for trial and optimisation.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: Nonidet® P 40 Substitute (NP-40) 10% in aqueous solution

Catalog Number: (BC96)

Supplier: G-Biosciences

Description: SATA reacts with primary amines, adding protected sulphhydryl residues. It is soluble in DMSO and the sulphhydryl group can be used in coupling reactions.

UOM: 1 * 100 mg

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Supplier: G-Biosciences

Description: Protein preparations specifically prepared for standardising electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (<50 μs). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic protein sample controls are available for animal cells, <i>E.coli</i>, yeast, plant or as a control set that contains one 2 mg vial of each.

Catalog Number: (786-224)

Supplier: G-Biosciences

Description: Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.

UOM: 1 * 50 mL

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Catalog Number: (786-226)

Supplier: G-Biosciences

Description: Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimise reoxidation of the competing thiol pairs as proteins enter into the second dimension gel. Improves resolution and prevents streaking of protein spots on 2D gels.

UOM: 1 * 50 mL

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Catalog Number: (786-R38)

Supplier: G-Biosciences

Description: Anti-IgG Goat Antibody (HRP (Horseradish Peroxidase))

UOM: 1 * 2 mL

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Supplier: G-Biosciences

Description: Tube-O-CONCENTRATOR™ Mini for 20 to 250 μl volumes or Medi for 0,2 to 2,5 ml volumes.

Catalog Number: (BE-501)

Supplier: G-Biosciences

Description: This lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how this highly specific interaction can be exploited as a tool for research and analysis. This study involves the use of an immunodiffusion technique in which antigen and antibody are allowed to diffuse in a solid agarose medium. When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pairs reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody. This Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of antigen-antibody interactions concepts such as immunoelectrophoresis and immunoprecipitation.

UOM: 1 * 1 KIT

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Catalog Number: (BE-312)

Supplier: G-Biosciences

Description: The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: ProteCEASE™ is a dry format version of ProteaseArrest™ for large scale preparative applications and for those users who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.

Supplier: G-Biosciences

Description: FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.

Supplier: G-Biosciences

Description: Carboxyl coupling resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.

Supplier: G-Biosciences

Description: The HOOK™ Peptide Coupling kits allow researchers to attach peptides to a carrier protein of choice using either primary amines or sulfhydryls in their peptide. The HOOK™ Peptide Coupling kits can be supplied with or without carrier proteins.