You Searched For: Chloroform

Catalog Number: (ICNA04856126)

Supplier: MP Biomedicals

Description: DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Useful for stabilizing sulfhydryl-containing enzymes.

UOM: 1 * 5 g

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Catalog Number: (D6943-03)

Supplier: OMEGA BIO-TEK

Description: Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.

UOM: 1 * 1 KIT

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Supplier: OMEGA BIO-TEK

Description: Isolate total RNA from cells or soft tissues using spin column.

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Supplier: MP Biomedicals

Description: Protein kinase C inhibitor (IC50 = 50-200 µM depending on assay conditions in MCF-7 cells or IC50 = 100 µM in rat brain). The PKC inhibition is also dependent on the phosopholipid concentration. Also inhibits both calmodulin-dependent and calmodulin-independent Ca2+-, Mg2+-ATPase. Tamoxifen is a selective estrogen response modifier (SERM), anti-angiogenetic factor. It is a prodrug that is metabolized to active metabolites 4-hydroxytamoxifen (4-OHT) and endoxifen by cytochrome P450 isoforms CYP2D6 and CYP3A4. In breast cancer, the gene repressor activity of tamoxifen against ERBB2 is dependent upon PAX2. Blocks estradiol-stimulated VEGF production in breast tumor cells.
Tamoxifen has been used to facilitate the recombination of ect2flox allele in mouse organs. It has also been used to study its effect on lipopolysaccharide (LPS)-induced microglial activation
Tamoxifen is a Protein kinase C inhibitor. It induces apoptosis in human malignant glioma cell lines. Tamoxifen and its metabolite 4-hydroxytamoxifen are selective estrogen response modifiers (SERMs) that act as estrogen antagonists in mammary gland. Blocks estradiol-stimulated VEGF production in breast tumor cells.

Catalog Number: (D6942-03)

Supplier: OMEGA BIO-TEK

Description: Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.

UOM: 1 * 1 KIT

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Catalog Number: (13-6834-02)

Supplier: VWR Chemicals

Description: Easy to use method for isolation up to 100 µg of total RNA from cultured eukaryotic cells and soft tissues <1×10⁷ cells or 30 mg tissue.

UOM: 1 * 200 Tests

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Catalog Number: (30-2010)

Supplier: VWR Chemicals

Description: TriFast™ is a complete ready to use reagent for simultaneous isolation of RNA, DNA and proteins from liquid samples, tissue and cells.

UOM: 1 * 100 mL

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Supplier: MP Biomedicals

Description: Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.

Supplier: ENZO LIFE SCIENCES

Description: EPIXTRACT® DNA isolation kit provides a simple method to isolate DNA from plasma, serum, and body fluids.

Catalog Number: (13-6945-00)

Supplier: VWR Chemicals

Description: Isolate up to 40 to 70 µg of high quality plasmid DNA from up to 15 ml bacterial cultures in 30 minutes or less. Simplified with VWR® peqGold Mini Column II technology into three quick steps: Bind, wash and elute.

UOM: 1 * 5 Tests

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Catalog Number: (N788-KIT)

Supplier: VWR Chemicals

Description: The phenol-free total RNA purification kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses.

UOM: 1 * 1 KIT

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Supplier: OMEGA BIO-TEK

Description: By adapting HiBind® technology with 96-well plates, the E-Z 96® Tissue DNA kit provides a high throughput method to purify genomic DNA from whole blood, buccal swabs, mouse tail, rat tail, tissues and animal cells and tissues in a 96-well plate format. Purified DNA is suitable for most downstream applications such as PCR, restriction enzyme digestion, and hybridisation techniques.

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Supplier: OMEGA BIO-TEK

Description: Isolate DNA from molluscs and insects using spin columns.

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Supplier: Quantabio

Description: Rapid extraction and purification of high-quality total DNA from cultured cells or tissue.

Catalog Number: (116541050.)

Supplier: MP Biomedicals

Description: Quick and convenient purification of total RNA from gram-positive and gram-negative bacteria using spin column technology.

UOM: 1 * 50 Tests

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Catalog Number: (ICNA116035050)

Supplier: MP Biomedicals

Description: FastRNA® Pro Red Kit is used to isolate total RNA from yeast and fungi. Isolated RNA is suitable for RT-PCR analysis and other downstream applications

UOM: 1 * 50 Tests

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