You Searched For: Renin+Substrate+1

Supplier: ENZO LIFE SCIENCES

Description: Caspase-1 substrate

Catalog Number: (10103.)

Supplier: Biotium

Description: Luciferin is a substrate for the enzyme firefly luciferase.

UOM: 1 * 5 mg

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Catalog Number: (ENZOBMLSE2110025)

Supplier: ENZO LIFE SCIENCES

Description: High purity industry standard substrate for deubiquitinylating enzymes (DUBs).

UOM: 1 * 25 µG

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Supplier: G-Biosciences

Description: The femtoELISA™ kits utilise a non animal protein blocker, NAP-BLOCKER™ that minimises cross-reactivity with researcher's antigens and antibodies. For the detection of HRP, an improved, ultra sensitive, non volatile, stable colorimetric substrate based on tetramethyl benzidine (TMB) is supplied. Reagent does not require hydrogen peroxide that can have detrimental effects on many biological factors, which may ultimately affect the assays.

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Catalog Number: (ENZOBMLP3150500)

Supplier: ENZO LIFE SCIENCES

Description: Insulin receptor substrate

UOM: 1 * 0,5 mg

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Catalog Number: (CAYM601010-1)

Supplier: Cayman Chemical

Description: For the measurement of NET-derived neutrophil elastase.

UOM: 1 * 1 items

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Catalog Number: (90A1-1KT)

Supplier: SIGMA ALDRICH MICROSCOPY

Description: Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates.

UOM: 1 * 1 KIT

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Catalog Number: (ENZOBMLP1640001)

Supplier: ENZO LIFE SCIENCES

Description: VHR substrate

UOM: 1 * 1 mg

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Catalog Number: (P0425-72EA)

Supplier: SIGMA ALDRICH MICROSCOPY

Description: Poly-prep slides are made of glass and designed for cell adhesion. The slides are coated with Poly-L-Lysine, a non-specific attachment factor for cells useful in promoting cell adhesion on solid substrates. Poly-L-lysine enhances binding between the cell membrane and the cultured surface.

UOM: 1 * 72 items

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Supplier: ENZO LIFE SCIENCES

Description: The matrix metalloproteinase (MMP) RED and GREEN Drug Discovery Kits are complete assay systems designed to screen MMP inhibitors, using long-wavelength quenched fluorogenic substrates OmniMMP™ RED or Fluorogenic Substrate (GREEN). The assays are performed in a convenient 96-well microplate format, with the compound NNGH also included as a prototypic control inhibitor.

Catalog Number: (BOSSBS-1135R-CY3)

Supplier: Bioss

Description: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

UOM: 1 * 100 µl

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Catalog Number: (ENZOENZ42809)

Supplier: ENZO LIFE SCIENCES

Description: Bio-N⁶-ddATP (Biotin-N⁶-(2’,3’-dideoxy)adenosine-5’ triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-N⁶-ddAMP. It is also a substrate for Taq DNA Polymerase and other DNA polymerases.

UOM: 1 * 25 Nmo

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Catalog Number: (BOSSBS-1135R-CY7)

Supplier: Bioss

Description: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

UOM: 1 * 100 µl

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Catalog Number: (BOSSBS-1135R-FITC)

Supplier: Bioss

Description: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

UOM: 1 * 100 µl

Sale

Catalog Number: (ENZOADI800076)

Supplier: ENZO LIFE SCIENCES

Description: AP substrate.

UOM: 1 * 100 mL

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate